circRNA basic information
circBase ID: -
Name: hsa_circ_SETD3
Synonym: circSETD3
Host Gene: SETD3
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004986
MONDO name: urinary bladder carcinoma
Disease details: bladder cancer
Disease DO ID:
 4007
Disease MeSH ID:
 -
Disease NCIt ID:
 C4912
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BLCA tissues; adjacent non-tumor tissues; cancerous clinical tissues
Cell lines:

HCV29; T24; J87; SW780; UMUC3; 293 T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: PTEN / PTEN mRNA / Ago2
Associated microRNA: miR-641
Biological function: Acts as a tumor suppressor; suppresses BLCA cell proliferation, cell viability, colony formation, migration, invasion, EMT process, stemness maintenance, sphere formation, and in vivo tumor growth.
Molecular mechanism: circSETD3 functions as a miRNA sponge for miR-641, thereby upregulating PTEN and inhibiting BLCA progression.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); EMT (inhibits); stemness (inhibits); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; Nuclear-Cytoplasmic Fractionation; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Low circSETD3 expression is associated with larger tumor size, advanced clinical stages, positive lymph metastasis and worse prognosis; high circSETD3 expression is associated with optimistic survival time and may serve as a potential diagnostic and therapeutic molecular marker for BLCA.
Description:

circSETD3 is down-regulated in bladder cancer tissues and cell lines, and higher circSETD3 expression is associated with better patient survival. Functionally, circSETD3 suppresses BLCA cell growth, migration, invasion, EMT, stemness maintenance, and xenograft tumor growth. Mechanistically, it acts as a cytoplasmic ceRNA sponge for miR-641 to increase PTEN expression.
Confidence score:

0.8439
Other information
Title:

Circular RNA circSETD3 hampers cell growth, migration, and stem cell properties in bladder cancer through sponging miR-641 to upregulate PTEN.

Journal: Cell cycle (Georgetown, Tex.)
Published: 2021
PubMed ID: 34288821
Study type:

combined biological and clinical study
Data availability: -
Code availability: -