circRNA basic information
circBase ID: hsa_circ_0007031
Name: hsa_circ_TUBGCP3
Synonym: circ_0007031
Host Gene: TUBGCP3
Genomic location(hg19): chr13:113158345-113181798:-
Genomic location(hg38): chr13:112504031-112527484:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
5672, 9256
Disease MeSH ID:
-
Disease NCIt ID:
C4978
Disease ICD11 ID:
-
Disease OMIM ID:
114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tumor tissues; adjacent normal tissues

Cell lines:

HCT116; Caco2; NCM460; HCT116/CRR; Caco2/CRR

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: DCP1A
Associated microRNA: miR-760
Biological function: promotes proliferation, chemoradiotherapy resistance, and tumor growth; inhibits apoptosis; promotes cell cycle progression
Molecular mechanism: ceRNA mechanism: circ_0007031 sponges miR-760 to derepress DCP1A, promoting CRC growth and chemoradiotherapy resistance
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); cell cycle (promotes); chemoresistance (promotes); radioresistance (promotes); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; Clinical Sample Validation; In Vivo Animal Model

Clinical significance:

high expression of circ_0007031 was markedly related to the tumor size, TNM stage and CEA of CRC patients

Description:

circ_0007031 is up-regulated in colorectal cancer, enriched in the cytoplasm, and promotes proliferation and chemoradiotherapy resistance. Mechanistically, it sponges miR-760 to increase DCP1A expression, thereby enhancing 5-Fu and radiation resistance; silencing circ_0007031 increases treatment sensitivity in vitro and in xenograft tumors.

Confidence score:

0.7635

Other information
Title:

Circ_0007031 Serves as a Sponge of miR-760 to Regulate the Growth and Chemoradiotherapy Resistance of Colorectal Cancer via Regulating DCP1A.

Journal: Cancer management and research
Published: 2020
PubMed ID: 32982440
Study type:

combined biological and clinical study

Data availability: The analyzed data sets generated during the present study are available from the corresponding authors (Chao Li and Xiaosong Gu) upon reasonable request.
Code availability: -