| Expression pattern: |
UP |
| Associated gene: |
ARL2, MAPK1, PTEN |
| Associated microRNA: |
hsa-mir-214-3p, hsa-mir-761, hsa-mir-194-3p, hsa-mir-412-3p, hsa-mir-362-5p |
| Biological function: |
promotes CRC progression; promotes proliferation, migration, and invasion |
| Molecular mechanism: |
may act as a miRNA sponge to regulate a circRNA-miRNA-mRNA network; knockdown increases hsa-mir-214-3p |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); cell cycle (other); angiogenesis (other); EGFR (other); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Sanger Sequencing; RNase R Treatment; Back-Splice Junction PCR / divergent primers PCR; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; ROC Analysis; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
positively correlated with tumor stage and lymph node metastasis; ROC AUC 0.7274 for distinguishing CRC from adjacent normal tissues; potential biomarker/therapeutic target |
| Description: |
hsa_circ_0044556 is up-regulated in colorectal cancer tissues and is associated with advanced tumor stage and lymph node metastasis. Functionally, its knockdown suppresses CRC cell proliferation, migration, and invasion, suggesting an oncogenic role. Mechanistically, it was proposed to act as a miRNA sponge within a circRNA-miRNA-mRNA network, with hsa-mir-214-3p highlighted as inversely affected upon circRNA silencing. |
| Confidence score: |
0.7932 |