| Expression pattern: |
UP |
| Associated gene: |
CCND2, Ago2 |
| Associated microRNA: |
miR-186-5p |
| Biological function: |
Circ_0008360 aggravates high glucose-induced HUVEC dysfunction; circ_0008360 knockdown mitigates high glucose-induced suppression of viability, migration and tube formation and reduces apoptosis. |
| Molecular mechanism: |
Circ_0008360 acts as a ceRNA by sponging miR-186-5p to up-regulate CCND2, thereby modulating high glucose-induced vascular endothelial dysfunction. |
| Biological pathway or process: |
ceRNA regulation (other); apoptosis (promotes); migration (inhibits); angiogenesis (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Transfection; MTT; Annexin V/PI Flow Cytometry; Wound Healing Assay; Tube Formation Assay; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Circ_0008360 might act as a target for the treatment of vascular endothelial dysfunction-related disorders. |
| Description: |
Hsa_circ_0008360 is up-regulated in high glucose-treated HUVEC and aggravates vascular endothelial dysfunction by reducing viability, migration and tube formation while promoting apoptosis. Mechanistically, it sponges miR-186-5p to regulate CCND2 through a ceRNA-based circ_0008360/miR-186-5p/CCND2 axis, suggesting therapeutic potential for vascular endothelial dysfunction-related disorders. |
| Confidence score: |
0.721 |