| Expression pattern: |
UP |
| Associated gene: |
TPX2 microtubule nucleation factor, PI3K, AKT |
| Associated microRNA: |
miR-515-5p |
| Biological function: |
promotes cell viability, colony formation, migration, invasion, cell cycle progression, and glycolysis; inhibits apoptosis; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA mechanism: circ-LARP1B sponges miR-515-5p to up-regulate TPX2 and activate PI3K/AKT signaling |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); cell cycle (promotes); glycolysis (promotes); apoptosis (inhibits); PI3K/AKT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; MTT; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
High circ-LARP1B expression was closely associated with positive lymph node metastasis and poor histopathological grade. |
| Description: |
circ-LARP1B is up-regulated in cutaneous squamous cell carcinoma and promotes malignant phenotypes (proliferation/colony formation, migration/invasion, cell-cycle progression, and glycolysis) while inhibiting apoptosis. Mechanistically, it acts as a ceRNA by sponging miR-515-5p to increase TPX2 expression and activate PI3K/AKT signaling, and its knockdown suppresses xenograft tumor growth. |
| Confidence score: |
0.8039 |