| Expression pattern: |
DN |
| Associated gene: |
SIRT6 |
| Associated microRNA: |
miR-33a-5p |
| Biological function: |
circ-ITCH overexpression inhibited viability, migration, fibrosis and inflammatory response of HG-induced RMCs and ameliorated renal inflammation and fibrosis in STZ-induced diabetic mice. |
| Molecular mechanism: |
circ-ITCH acts through sponge adsorption of miR-33a-5p to modulate SIRT6 expression. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); fibrosis (inhibits); inflammation (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Wound Healing Assay; IF (Immunofluorescence); ELISA; Western Blot; Nuclear-Cytoplasmic Fractionation; In Vivo Animal Model; H&E Staining |
| Clinical significance: |
- |
| Description: |
circ-ITCH was downregulated in high-glucose-induced RMCs. Its overexpression suppressed RMC viability, migration, fibrosis and inflammatory responses, and improved renal inflammation and fibrosis in STZ-induced diabetic mice. Mechanistically, circ-ITCH acted as a sponge for miR-33a-5p to regulate SIRT6 expression. |
| Confidence score: |
0.6006 |