circRNA basic information
circBase ID: hsa_circ_0055033
Name: hsa_circ_ANTXR1
Synonym: circANTXR1
Host Gene: ANTXR1
Genomic location(hg19): chr2:69304539-69318051:+
Genomic location(hg38): chr2:69077407-69090919:+
Subcellular localization: exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
684, 686
Disease MeSH ID:
D006528
Disease NCIt ID:
C3099
Disease ICD11 ID:
1294035808
Disease OMIM ID:
114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; paracancerous normal tissues; serum; serum exosomes; exosomes

Cell lines:

HuH-7; HCCLM3; THLE-2

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: XRCC5
Associated microRNA: miR-532-5p
Biological function: promotes proliferation, migration and invasion; promotes tumor growth; promotes metastasis
Molecular mechanism: Acts as a miRNA sponge for miR-532-5p to upregulate XRCC5 (ceRNA axis); transported via exosomes
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Clinical Sample Validation; Survival Analysis; ROC Analysis; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; Transfection; MTT; EdU Staining; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry)

Clinical significance:

High circANTXR1 expression was associated with lower overall survival; serum exosome circANTXR1 showed diagnostic significance (AUC=0.76).

Description:

circANTXR1 is up-regulated in HCC tissues/cells and promotes proliferation and metastasis. Mechanistically, it sponges miR-532-5p to increase XRCC5 expression, and it is transported via exosomes; serum exosomal circANTXR1 shows diagnostic potential and high tumor expression predicts worse survival.

Confidence score:

0.8283

Other information
Title:

CircANTXR1 Contributes to the Malignant Progression of Hepatocellular Carcinoma by Promoting Proliferation and Metastasis.

Journal: Journal of hepatocellular carcinoma
Published: 2021
PubMed ID: 34786378
Study type:

combined biological and clinical study

Data availability: GSE97332; GSE78520
Code availability: -