circRNA basic information
circBase ID: -
Name: hsa_circ_HIPK3
Synonym: -
Host Gene: HIPK3
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BCa tissues; adjacent non-cancerous tissues (ANT); tumor tissues
Cell lines:

MCF-10A; MCF7; SK-BR-3; BT549; BT20; MDA-MB-231; MDA-MB-453
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: Bcl-2, Bax, cleaved Caspase-3, E-cadherin, N-cadherin, Vimentin, Ki-67
Associated microRNA: miR-326
Biological function: promotes proliferation, colony formation, migration, invasion, apoptosis resistance, tumor growth, tumorigenicity and metastasis in breast cancer
Molecular mechanism: circHIPK3 functions as a miR-326 sponge; circHIPK3 regulates apoptotic molecules and EMT-related markers including Bcl-2, Bax, cleaved Caspase-3, E-Cadherin, N-Cadherin and Vimentin
Biological pathway or process:

ceRNA regulation (other); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); EMT (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis
Clinical significance:

potential therapeutic target for treating BCa
Description:

circHIPK3 is up-regulated in breast cancer tissues and cell lines and acts as an oncogenic circRNA. It promotes proliferation, migration, invasion, EMT-like marker changes, apoptosis resistance and xenograft tumor growth by sponging miR-326 and regulating apoptosis- and metastasis-related proteins. The study proposes circHIPK3 as a potential therapeutic target for breast cancer.
Confidence score:

0.8175
Other information
Title:

Circular RNA circHIPK3 promotes breast cancer progression via sponging MiR-326.

Journal: Cell cycle (Georgetown, Tex.)
Published: 2021
PubMed ID: 34152928
Study type:

combined biological and clinical study
Data availability: The analyzed data sets generated during the study are available from the corresponding author on reasonable request.
Code availability: -