| Expression pattern: |
UP |
| Associated gene: |
RAB14, GPX4, xCT |
| Associated microRNA: |
miR-188-5p |
| Biological function: |
circ-IARS promotes osteosarcoma cell proliferation and invasion, suppresses ferroptosis, promotes tumor growth in vivo, and is associated with clinical stage, distant metastasis, and reduced overall survival. |
| Molecular mechanism: |
circ-IARS acts as a ceRNA/miRNA sponge for miR-188-5p to positively regulate RAB14 expression, thereby promoting OS cell proliferation and invasion and inhibiting ferroptosis-related effects. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); ferroptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; CCK8; Transwell Assay; IF (Immunofluorescence); Western Blot; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis |
| Clinical significance: |
circ-IARS expression is closely related to clinical stage and distant metastasis, and high circ-IARS levels are associated with reduced overall survival in OS patients. |
| Description: |
circ-IARS is up-regulated in osteosarcoma tissues and cells and functions as an oncogenic circRNA. It promotes OS proliferation, invasion, and in vivo tumor growth while suppressing ferroptosis through the miR-188-5p/RAB14 regulatory axis. High circ-IARS expression is associated with advanced clinical stage, distant metastasis, and poorer overall survival. |
| Confidence score: |
0.7972 |