| Expression pattern: |
UP |
| Associated gene: |
IL-10 |
| Associated microRNA: |
miR-125a-3p |
| Biological function: |
circMERTK enhances the immunosuppressive activity of TAM-like cells and promotes apoptosis of CD8+ T cells in the colorectal cancer microenvironment. |
| Molecular mechanism: |
circMERTK acts as a sponge for miR-125a-3p, thereby upregulating IL-10 expression and promoting TAM-like cell-mediated immunosuppressive activity. |
| Biological pathway or process: |
apoptosis (promotes); immune regulation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; RT-qPCR; ISH (In Situ Hybridization); IF (Immunofluorescence); Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; Annexin V/PI Flow Cytometry; ELISA; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circMERTK may serve as a potential therapeutic target for CRC. |
| Description: |
circMERTK is up-regulated in TAM-like cells and CRC tissues, especially overlapping with CD68-positive macrophages. It promotes TAM-like cell immunosuppressive activity by sponging miR-125a-3p to increase IL-10, thereby enhancing CD8+ T-cell apoptosis, and may represent a potential therapeutic target in CRC. |
| Confidence score: |
0.8331 |