| Expression pattern: |
UP |
| Associated gene: |
YY1, Ago2 |
| Associated microRNA: |
miR-211-5p |
| Biological function: |
Promotes GBM cell proliferation, colony formation, migration, invasion and tumor growth in vivo; knockdown suppresses these phenotypes. |
| Molecular mechanism: |
circ_0001588 acts as a cytoplasmic ceRNA that sponges miR-211-5p and positively regulates YY1 expression, thereby facilitating GBM progression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Actinomycin D / DRB Stability Assay; RNase R Treatment; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_0001588 expression is associated with lower survival and may act as a prognostic biomarker or target for GBM diagnosis and treatment. |
| Description: |
circ_0001588 is up-regulated in GBM tissues and glioma cell lines, and high expression is associated with poor patient survival. Functionally, circ_0001588 promotes GBM proliferation, migration, invasion and xenograft tumor growth. Mechanistically, it acts as a cytoplasmic ceRNA that sponges miR-211-5p to increase YY1 expression. |
| Confidence score: |
0.8768 |