| Expression pattern: |
UP |
| Associated gene: |
TRIM44 |
| Associated microRNA: |
miR-384 |
| Biological function: |
Promotes NSCLC cell viability, migration, invasion and EMT; inhibits apoptosis; promotes tumor growth in vivo. |
| Molecular mechanism: |
Acts as a ceRNA by sponging miR-384, relieving miR-384-mediated repression of TRIM44 (circ_0020123/miR-384/TRIM44 axis). |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; MTT; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_0020123 expression was negatively correlated with the overall survival rate of patients; suggested as a novel biomarker for NSCLC. |
| Description: |
circ_0020123 is up-regulated in NSCLC tissues and cell lines and promotes malignant phenotypes (viability, migration, invasion, EMT) while inhibiting apoptosis. Mechanistically, it sponges miR-384 to increase TRIM44 expression (circ_0020123/miR-384/TRIM44 axis), and its knockdown suppresses xenograft tumor growth; high circ_0020123 is associated with worse overall survival. |
| Confidence score: |
0.8347 |