circRNA basic information
circBase ID: -
Name: hsa_circ_NOLC1
Synonym: circNOLC1
Host Gene: -
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

breast cancer tissues; adjacent nontumor tissues
Cell lines:

MDA-MB-231; MDA-MB-468
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: STAT3
Associated microRNA: miR-365a-3p
Biological function: Promotes breast cancer cell proliferation/viability, migration/invasion, EMT and cancer stem cell properties; mediates propofol effects on tumor growth.
Molecular mechanism: Acts as a ceRNA sponge for miR-365a-3p to enhance STAT3 expression; STAT3 binds circNOLC1 promoter and propofol inhibits this STAT3-dependent transcription in a feedback loop.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); stemness (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Western Blot; Flow Cytometry（Non-apoptosis/cycle）; Luciferase Reporter Assay; RNA Pull-Down; ChIP / ChIP-seq; In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

-
Description:

circNOLC1 is up-regulated in breast cancer and promotes proliferation, migration/invasion, EMT, and cancer stem cell properties. Mechanistically, it acts as a ceRNA for miR-365a-3p to increase STAT3, and propofol suppresses circNOLC1 via inhibiting STAT3 recruitment to the circNOLC1 promoter, with circNOLC1 overexpression rescuing propofol’s anti-tumor effects in vitro and in vivo.
Confidence score:

0.7589
Other information
Title:

The inhibition of circular RNA circNOLC1 by propofol/STAT3 attenuates breast cancer stem cells function via miR-365a-3p/STAT3 signaling.

Journal: Journal of translational medicine
Published: 2021
PubMed ID: 34789263
Study type:

combined biological and clinical study
Data availability: The datasets used and analysed during the current study are available from the corresponding author on reasonable request.
Code availability: -