| Expression pattern: |
UP |
| Associated gene: |
DNMT3A |
| Associated microRNA: |
miR-599 |
| Biological function: |
Circ_0084615 promotes CRC cell proliferation, migration, invasion and metastatic potential; knockdown of circ_0084615 restrains these malignant phenotypes. |
| Molecular mechanism: |
Circ_0084615 functions as a competing endogenous RNA by sponging miR-599, thereby relieving miR-599-mediated repression of DNMT3A and increasing DNMT3A expression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Western Blot; In Vivo Animal Model; H&E Staining; Survival Analysis |
| Clinical significance: |
Circ_0084615 up-regulation is associated with poor overall survival, higher TNM stage, and may be a prognostic biomarker and therapeutic target for CRC. |
| Description: |
Circ_0084615 is an ASPH-derived human circRNA up-regulated in colorectal cancer tissues and cell lines. It promotes CRC proliferation, migration, invasion and lung metastatic colonization by acting as a ceRNA that sponges miR-599 and increases DNMT3A expression. Clinically, high circ_0084615 expression is associated with worse overall survival and higher TNM stage, suggesting biomarker and therapeutic-target potential. |
| Confidence score: |
0.7486 |