| Expression pattern: |
UP |
| Associated gene: |
Snail, dipeptidyl peptidase 4(DPP4), CXCL10 |
| Associated microRNA: |
miR-30a-5p, miR-30b-5p, miR-30c-5p, miR-30d-5p, miR-30e-5p |
| Biological function: |
Promotes HCC invasion and metastasis, induces EMT, and promotes an immunosuppressive tumor microenvironment with reduced CD8+ T cell infiltration; contributes to anti-PD1 therapy resistance. |
| Molecular mechanism: |
Acts as a miRNA sponge for the miR-30-5p family, relieving repression of Snail; Snail transcriptionally upregulates DPP4, which reduces CXCL10 and limits CD8+ T cell trafficking, creating immunosuppression and resistance to anti-PD1 therapy. |
| Biological pathway or process: |
EMT (promotes); invasion (promotes); metastasis (promotes); immune regulation (inhibits); chemokine signaling pathway (other); PI3K/AKT (other); ceRNA regulation (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
RT-qPCR; ISH (In Situ Hybridization); Clinical Sample Validation; Cohort Study; Survival Analysis; circRIP; RNA Pull-Down; Luciferase Reporter Assay; FISH / smFISH; Transfection; Transwell Assay; Wound Healing Assay; Colony Formation Assay; MTT; Cell Cycle Assay; RNA-seq; ChIP / ChIP-seq; ELISA; IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
High circMET expression is associated with poor overall survival and higher cumulative recurrence rates; circMET expression is an independent predictor of OS and postoperative recurrence in HCC. |
| Description: |
circMET (hsa_circ_0082002), derived from MET, is up-regulated in HCC and predicts worse survival and higher recurrence. Functionally, circMET promotes invasion/metastasis and EMT while suppressing antitumor immunity by sponging the miR-30-5p family to increase Snail and downstream DPP4, reducing CXCL10 and CD8+ T-cell infiltration, contributing to anti-PD1 resistance. |
| Confidence score: |
0.8283 |