| Expression pattern: |
UP |
| Associated gene: |
TLR4, NLRP3, p-p65, p-IkappaBalpha, C-Caspase-1, IL-1beta, TNF-alpha |
| Associated microRNA: |
miR-192, miR-561 |
| Biological function: |
Promotes inflammatory cytokine production and inflammatory response in gouty arthritis; circHIPK3 knockdown suppresses gouty arthritis in vivo. |
| Molecular mechanism: |
circHIPK3 acts as a miRNA sponge for miR-192 and miR-561, thereby promoting TLR4 and NLRP3 expression and activating inflammatory signaling pathways. |
| Biological pathway or process: |
NF-kappaB (promotes); inflammation (promotes); immune regulation (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; RNA-seq; Clinical Sample Validation; Transfection; ELISA; Western Blot; RNA Pull-Down; FISH / smFISH; Luciferase Reporter Assay; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
circHIPK3 was elevated in SFMCs from patients with gouty arthritis, positively correlated with serum uric acid level, and may be a promising treatment target. |
| Description: |
circHIPK3 is up-regulated in SFMCs from patients with gouty arthritis and in MSU-stimulated THP-1 cells. It promotes inflammatory cytokine production and inflammatory signaling by sponging miR-192 and miR-561, thereby increasing TLR4 and NLRP3 expression. Knockdown of circHIPK3 suppresses gouty arthritis in an MSU-induced mouse model, suggesting therapeutic potential. |
| Confidence score: |
0.7372 |