| Expression pattern: |
DN |
| Associated gene: |
WNK2 |
| Associated microRNA: |
miR-620 |
| Biological function: |
Circ_0001666 acts as a tumor suppressor in breast cancer, inhibiting cell proliferation, migration, invasion, metastasis-related phenotypes and tumor growth, while promoting apoptosis and inducing G0/G1 cell-cycle arrest. |
| Molecular mechanism: |
Circ_0001666 functions through a ceRNA mechanism by sponging miR-620, thereby positively regulating WNK2 expression and inhibiting breast cancer progression. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); apoptosis (promotes); cell cycle (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model |
| Clinical significance: |
circ_0001666 expression was related to tumor size, TNM stage and lymph node metastases in breast cancer patients, and the authors proposed it as a new target for targeted therapy of breast cancer. |
| Description: |
This study identified circ_0001666 as a downregulated circRNA in breast cancer tissues and cell lines. Functional assays showed that circ_0001666 suppresses breast cancer progression by inhibiting proliferation, migration, invasion, metastasis-related phenotypes and tumor growth, while promoting apoptosis. Mechanistically, circ_0001666 acts as a miR-620 sponge to upregulate WNK2 in a circ_0001666/miR-620/WNK2 ceRNA axis. |
| Confidence score: |
0.7799 |