circRNA basic information
circBase ID: hsa_circ_0001955
Name: hsa_circ_CSNK1G1
Synonym: circ_0001955
Host Gene: CSNK1G1
Genomic location(hg19): chr15:64495280-64508912:-
Genomic location(hg38): chr15:64203081-64216713:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; adjacent normal tissues; xenograft tumor tissues
Cell lines:

SNU-387; Huh7; THLE-2; HUVECs
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: FZD4
Associated microRNA: miR-646
Biological function: promotes HCC cell proliferation, metastasis, angiogenesis and tumor growth; inhibits apoptosis
Molecular mechanism: circ_0001955 acts as a miR-646 sponge and promotes FZD4 expression through the miR-646/FZD4 axis
Biological pathway or process:

proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); metastasis (promotes); angiogenesis (promotes); apoptosis (inhibits); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; EdU Staining; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; Luciferase Reporter Assay; RNA Pull-Down; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

high expression was related to tumor size and AFP level of HCC patients; expression increased with HCC malignancy; potential therapeutic target for HCC
Description:

circ_0001955 is up-regulated in HCC tissues and cell lines and is associated with tumor size, AFP level, and increasing malignancy. Functionally, circ_0001955 promotes proliferation, cell-cycle progression, migration, invasion, angiogenesis, and in vivo tumor growth while inhibiting apoptosis. Mechanistically, it acts as a ceRNA for miR-646 to increase FZD4 expression, forming a circ_0001955/miR-646/FZD4 axis that supports HCC progression.
Confidence score:

0.8299
Other information
Title:

Circ_0001955 Acts as a miR-646 Sponge to Promote the Proliferation, Metastasis and Angiogenesis of Hepatocellular Carcinoma.

Journal: Digestive diseases and sciences
Published: 2022
PubMed ID: 34021822
Study type:

combined biological and clinical study
Data availability: -
Code availability: -