circRNA basic information
circBase ID: -
Name: hsa_circ_VIM
Synonym: circ-VIM
Host Gene: vimentin
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007576
MONDO name: esophageal cancer
Disease details: esophageal cancer
Disease DO ID:
 5041
Disease MeSH ID:
 -
Disease NCIt ID:
 C7478
Disease ICD11 ID:
 669033105
Disease OMIM ID:
 133239
Species: Human
Species details: Homo sapiens
Tissue specimen:

EC tissues; matching normal esophageal tissues; xenograft tumors; lung tissues
Cell lines:

HET-1A; KYSE-150; Eca-109; TE-10; TE-11; 293T; CD8 + T cells
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PD-L1, Ago2, Ras, ERK1/2, Slug, Fibronectin, E-cadherin, N-cadherin
Associated microRNA: miR-124-3p (miR-124)
Biological function: circ-VIM promotes EC cell viability, long-term proliferation, migration, invasion, EMT, xenograft growth, lung metastasis, and immune escape; silencing circ-VIM enhances CD8 + T-cell cytotoxicity and proliferation and inhibits CD8 + T-cell apoptosis.
Molecular mechanism: circ-VIM acts as a miR-124 sponge and releases miR-124-mediated suppression of PD-L1, thereby activating PD-L1-associated Ras/ERK signaling and EMT; circ-VIM silencing synergizes with sevoflurane to upregulate miR-124 and downregulate PD-L1.
Biological pathway or process:

ceRNA regulation (other); ERK (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); apoptosis (promotes); immune regulation (promotes); other pathway/process (other)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Flow Cytometry（Non-apoptosis/cycle）; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); IF (Immunofluorescence); H&E Staining
Clinical significance:

Higher circ-VIM expression correlated with tumors of advanced TNM stage and positive lymph node metastasis in EC patients.
Description:

circ-VIM is up-regulated in esophageal cancer tissues and cell lines, and higher expression is associated with advanced TNM stage and lymph node metastasis. Functionally, circ-VIM promotes EC proliferation, migration, invasion, EMT, metastasis, xenograft growth, and immune escape by sponging miR-124 and derepressing PD-L1, with downstream Ras/ERK signaling involvement. Silencing circ-VIM synergizes with sevoflurane to suppress malignant phenotypes and enhance CD8 + T-cell antitumor activity through the miR-124/PD-L1 axis.
Confidence score:

0.7554
Other information
Title:

CircRNA VIM silence synergizes with sevoflurane to inhibit immune escape and multiple oncogenic activities of esophageal cancer by simultaneously regulating miR-124/PD-L1 axis.

Journal: Cell biology and toxicology
Published: 2022
PubMed ID: 34018092
Study type:

combined biological and clinical study
Data availability: The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -