circRNA basic information
circBase ID: hsa_circ_0080223
Name: hsa_circ_EGFR
Synonym: circRNA epidermal growth factor receptor
Host Gene: EGFR
Genomic location(hg19): chr7:55221703-55241736:+
Genomic location(hg38): chr7:55154010-55174043:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0021042
MONDO name: glioma
Disease details: glioma
Disease DO ID:
 -
Disease MeSH ID:
 D005910
Disease NCIt ID:
 C3059
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

glioma samples; glioma tissues; normal brain tissues
Cell lines:

LN229; T98G; NHA
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: TUSC2
Associated microRNA: miR-183-5p
Biological function: Circ-EGFR inhibits glioma cell proliferation, colony formation, migration and invasion, promotes apoptosis, and reduces glioma tumor growth in vivo.
Molecular mechanism: Circ-EGFR acts as a sponge for miR-183-5p and promotes TUSC2 expression, thereby inhibiting glioma progression via the circ-EGFR/miR-183-5p/TUSC2 axis.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis
Clinical significance:

Circ-EGFR may be a useful therapeutic target to antagonize glioma progression.
Description:

Circ-EGFR is down-regulated in glioma tissues and glioma cell lines. Functional assays show that circ-EGFR overexpression suppresses proliferation, migration, invasion and tumor growth while promoting apoptosis. Mechanistically, circ-EGFR acts as a miR-183-5p sponge to increase TUSC2 expression, forming a tumor-inhibitory circ-EGFR/miR-183-5p/TUSC2 axis.
Confidence score:

0.6542
Other information
Title:

Circ-EGFR Functions as an Inhibitory Factor in the Malignant Progression of Glioma by Regulating the miR-183-5p/TUSC2 Axis.

Journal: Cellular and molecular neurobiology
Published: 2022
PubMed ID: 33993369
Study type:

combined biological and clinical study
Data availability: The datasets used and analyzed during the current study are available from the corresponding author on reasonable request; https://doi.org/10.1007/s10571-021-01099-y
Code availability: -