circRNA basic information
circBase ID: hsa_circ_0092283
Name: hsa_circ_MYH9
Synonym: circRNA myosin heavy chain 9
Host Gene: MYH9
Genomic location(hg19): chr22:36681395-36681695:-
Genomic location(hg38): chr22:36285349-36285649:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
5672, 9256
Disease MeSH ID:
-
Disease NCIt ID:
C4978
Disease ICD11 ID:
-
Disease OMIM ID:
114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC specimens; corresponding normal specimens; adjacent normal tissues; xenograft tumor tissues

Cell lines:

NCM460; HCT116; SW620

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: HDGF, Ago2
Associated microRNA: miR-761
Biological function: circMYH9 promotes CRC malignant behaviors, including cell viability, proliferation, cell cycle progression, migration and invasion, inhibits apoptosis, and counteracts Baicalin-induced anti-tumor effects.
Molecular mechanism: circMYH9 acts as a molecular sponge for miR-761 and up-regulates HDGF expression, forming a circMYH9/miR-761/HDGF ceRNA axis involved in Baicalin response in CRC.
Biological pathway or process:

proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); ceRNA regulation (other); drug resistance (other)

Detected method:
Q
Validation methods:

Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model

Clinical significance:

circMYH9 was abnormally upregulated in CRC tissues and was suggested as a stable circRNA biomarker candidate, but no diagnostic or prognostic performance was reported.

Description:

circMYH9 is up-regulated in colorectal cancer tissues and cell lines and is reduced by Baicalin treatment. Functionally, circMYH9 promotes malignant phenotypes and attenuates Baicalin-induced anti-tumor effects by sponging miR-761 and up-regulating HDGF in CRC cells.

Confidence score:

0.795

Other information
Title:

Use Chou's 5-steps rule to study how Baicalin suppresses the malignant phenotypes and induces the apoptosis of colorectal cancer cells.

Journal: Archives of biochemistry and biophysics
Published: 2021
PubMed ID: 33992597
Study type:

combined biological and clinical study

Data availability: GSE126094; TCGA
Code availability: -