| Expression pattern: |
DN |
| Associated gene: |
PDCD4 |
| Associated microRNA: |
miR-425-5p |
| Biological function: |
CircMKLN1 acts as a tumor inhibitor in RB; its overexpression represses RB cell proliferation, colony formation, migration and invasion in vitro, inhibits EMT-related changes, and hampers tumor growth in vivo. |
| Molecular mechanism: |
CircMKLN1 functions as a miR-425-5p sponge and elevates PDCD4 expression, thereby suppressing RB malignant behaviors. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); EMT (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High levels of circMKLN1 were related to a favorable outcome of RB patients; circMKLN1 may provide a potential prognostic marker and therapeutic target for RB patients. |
| Description: |
CircMKLN1 is down-regulated in retinoblastoma tissues and cells, and higher circMKLN1 expression is associated with better overall survival. Functionally, circMKLN1 suppresses RB cell proliferation, migration, invasion, EMT-related protein changes, and xenograft tumor growth. Mechanistically, circMKLN1 acts through the circMKLN1/miR-425-5p/PDCD4 axis by sponging miR-425-5p and increasing PDCD4 expression. |
| Confidence score: |
0.8439 |