| Expression pattern: |
UP |
| Associated gene: |
GRB10 |
| Associated microRNA: |
miR-5195-3p |
| Biological function: |
circ_0009910 promotes AML cell proliferation and cell cycle progression and inhibits apoptosis; knockdown suppresses proliferation and cell cycle progression while facilitating apoptosis. |
| Molecular mechanism: |
Exosome-transmitted circ_0009910 acts as a ceRNA sponge for miR-5195-3p to modulate GRB10 expression, thereby regulating AML cell proliferation, cell cycle and apoptosis. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Bioinformatics Analysis |
| Clinical significance: |
High circ_0009910 expression was significantly correlated with AML risk status and was associated with poor risk status; it may have potential diagnostic and prognostic value in myeloid leukemia. |
| Description: |
In AML, hsa_circ_0009910/circ_0009910 is up-regulated in bone marrow samples, AML cell lines, and AML cell-derived exosomes. It functions as an oncogenic exosomal circRNA that promotes proliferation and cell cycle progression while inhibiting apoptosis, at least partly through the circ_0009910/miR-5195-3p/GRB10 ceRNA axis. Clinically, higher circ_0009910 expression is associated with AML risk status and poor-risk disease. |
| Confidence score: |
0.7032 |