| Expression pattern: |
UP |
| Associated gene: |
EZH2, Ago2 |
| Associated microRNA: |
miR-144 |
| Biological function: |
Promotes CRC cell invasion and is associated with liver metastasis. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-144, relieving repression of EZH2 and increasing EZH2 expression. |
| Biological pathway or process: |
invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis; RNase R Treatment; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; Transwell Assay; Western Blot |
| Clinical significance: |
Increased expression of circ_0115744 could differentiate CRLM from CRC and may serve as a biomarker/fingerprint to predict liver metastasis. |
| Description: |
circ_0115744 is up-regulated in CRC, particularly in CRC with liver metastasis, and can distinguish CRLM from non-metastatic CRC using plasma-based risk score/ROC analysis. It promotes CRC cell invasion and metastasis by sponging miR-144, thereby relieving miR-144-mediated repression of EZH2 and increasing EZH2 expression. |
| Confidence score: |
0.824 |