| Expression pattern: |
DN |
| Associated gene: |
EZH2, EED, SUZ12, H3K27me3, RORA |
| Associated microRNA: |
- |
| Biological function: |
Suppresses gastric cancer cell proliferation, invasion, migration, cell cycle progression and metastasis, and enhances apoptosis; acts as a tumor suppressor. |
| Molecular mechanism: |
circGSK3B directly binds EZH2 (PRC2-related) in the nucleus, reduces EZH2/H3K27me3 occupancy at the RORA promoter, thereby increasing RORA expression and suppressing GC progression (linked to Wnt/beta-catenin signaling). |
| Biological pathway or process: |
proliferation (inhibits); invasion (inhibits); migration (inhibits); metastasis (inhibits); cell cycle (inhibits); apoptosis (promotes); Wnt/beta-catenin (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; divergent primers PCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; ChIP / ChIP-seq; Transfection; MTT; Colony Formation Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
Low circGSK3B expression was associated with tumor size, lymph node metastasis and TNM stage in GC patients. |
| Description: |
circGSK3B (hsa_circ_0003763) is down-regulated in gastric cancer tissues and cell lines and functions as a tumor suppressor. It localizes mainly in the nucleus and directly binds EZH2, reducing EZH2/H3K27me3 repression at the RORA promoter to increase RORA expression, thereby inhibiting Wnt/beta-catenin-related malignant phenotypes (proliferation, invasion/migration, metastasis) and promoting apoptosis. |
| Confidence score: |
0.8568 |