| Expression pattern: |
UP |
| Associated gene: |
PDCD4, NF-kappaB signaling pathway, Ago2, p-IkappaBalpha, p-p65 |
| Associated microRNA: |
miR-326 |
| Biological function: |
circ-UQCRC2 knockdown attenuated LPS-induced MRC-5 cell injury by improving cell viability, reducing apoptosis, suppressing pro-inflammatory cytokine production, increasing IL-10, and inhibiting fibrosis-related markers. |
| Molecular mechanism: |
circ-UQCRC2 acts as a miR-326-targeting ceRNA/post-transcriptional regulator to increase PDCD4 expression and activate the NF-kappaB signaling pathway in LPS-treated MRC-5 cells. |
| Biological pathway or process: |
NF-kappaB (promotes); apoptosis (promotes); inflammation (promotes); fibrosis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; ELISA; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circ-UQCRC2 level was closely correlated with disease severity, tachypnea physical feature, and serum C-reactive protein concentration in pediatric pneumonia patients. |
| Description: |
circ-UQCRC2 is up-regulated in pediatric pneumonia serum and LPS-treated human MRC-5 lung fibroblastic cells. Functionally, circ-UQCRC2 promotes LPS-induced inflammatory injury, apoptosis, and NF-kappaB activation, while its knockdown alleviates cell injury. Mechanistically, circ-UQCRC2 directly targets miR-326 to regulate PDCD4 and the NF-kappaB pathway, and its expression correlates with clinical severity features in pediatric pneumonia. |
| Confidence score: |
0.7581 |