| Expression pattern: |
UP |
| Associated gene: |
SNCA, AGO2, JAK2, STAT3 |
| Associated microRNA: |
miR-7-5p |
| Biological function: |
circZNF208 promotes proliferation and X-ray radioresistance in NSCLC cells; knockdown of circZNF208 inhibits proliferation and enhances sensitivity to X-rays, but does not significantly alter sensitivity to carbon ions. |
| Molecular mechanism: |
circZNF208 acts as a competing endogenous RNA by sponging miR-7-5p, upregulates SNCA, and is linked to activation of the JAK2-STAT3 signalling pathway to regulate X-ray radiosensitivity in NSCLC cells. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); apoptosis (inhibits); JAK/STAT (promotes); radioresistance (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circZNF208 might serve as a potential biomarker and therapeutic target for selecting radiotherapy modalities in NSCLC. |
| Description: |
In NSCLC radioresistant A549-R11 cells, circZNF208 is upregulated and promotes resistance to low-LET X-ray irradiation. It functions as a ceRNA sponge for miR-7-5p, leading to increased SNCA expression and activation of the JAK2-STAT3-related pathway. circZNF208 knockdown sensitizes NSCLC cells to X-rays but not to carbon-ion irradiation, suggesting potential value as a biomarker for radiotherapy modality selection. |
| Confidence score: |
0.8161 |