circRNA basic information
circBase ID: hsa_circ_0020093
Name: hsa_circ_ATRNL1
Synonym: circ_0020093
Host Gene: ATRNL1
Genomic location(hg19): chr10:116879948-116889297:+
Genomic location(hg38): chr10:115120184-115129535:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005178
MONDO name: osteoarthritis
Disease details: Osteoarthritis
Disease DO ID:
 8398
Disease MeSH ID:
 D010003
Disease NCIt ID:
 C3293
Disease ICD11 ID:
 558562409
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

human chondrocytes
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: SPRY1
Associated microRNA: miR-23b
Biological function: ameliorates IL-1beta-induced apoptosis and extracellular matrix degradation in chondrocytes
Molecular mechanism: circ_0020093 acts as a miRNA sponge for miR-23b, thereby upregulating SPRY1
Biological pathway or process:

apoptosis (inhibits); extracellular matrix degradation (inhibits); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; Transfection; Annexin V/PI Flow Cytometry; Western Blot
Clinical significance:

-
Description:

In an IL-1beta-induced osteoarthritis-like human chondrocyte model, circ_0020093 is down-regulated and functions protectively by reducing apoptosis and extracellular matrix degradation. Mechanistically, circ_0020093 sponges miR-23b to relieve repression of SPRY1, forming a circ_0020093/miR-23b/SPRY1 axis.
Confidence score:

0.6089
Other information
Title:

Circ_0020093 ameliorates IL-1beta-induced apoptosis and extracellular matrix degradation of human chondrocytes by upregulating SPRY1 via targeting miR-23b.

Journal: Molecular and cellular biochemistry
Published: 2021
PubMed ID: 34046827
Study type:

biological research
Data availability: The analyzed data sets generated during the present study are available from the corresponding author on reasonable request.
Code availability: -