| Expression pattern: |
UP |
| Associated gene: |
GDPD5, DOCK1 |
| Associated microRNA: |
miR-874-3p |
| Biological function: |
Circ_0007142 promotes CRC cell proliferation and tumorigenesis, inhibits apoptosis, and suppresses ferroptosis; its knockdown reduces proliferation and tumour growth while promoting apoptosis and ferroptosis. |
| Molecular mechanism: |
Circ_0007142 acts as a miR-874-3p sponge to positively regulate GDPD5, thereby modulating CRC progression, tumorigenesis and ferroptosis. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); ferroptosis (inhibits); ceRNA regulation (other); mitochondrial function (other); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot; Bioinformatics Analysis; In Vivo Animal Model |
| Clinical significance: |
High circ_0007142 expression was associated with tumour size, TNM classification, distant metastasis and lymph node metastasis; circ_0007142 might be an available marker for ferroptosis in CRC therapy. |
| Description: |
Circ_0007142 is up-regulated in CRC tissues and cells and functions as an oncogenic circRNA. It sponges miR-874-3p to increase GDPD5 expression, thereby promoting CRC proliferation and tumorigenesis while inhibiting apoptosis and ferroptosis. Knockdown of circ_0007142 suppresses tumour growth in a cell line-derived xenograft model and may improve CRC therapy by enhancing ferroptosis. |
| Confidence score: |
0.7404 |