| Expression pattern: |
UP |
| Associated gene: |
EGFR, CCNE1, PIK3CD, RAF1, N-cadherin, E-cadherin, PCNA |
| Associated microRNA: |
miR-7 |
| Biological function: |
Promotes osteosarcoma tumor growth, cell viability and migration; inhibits apoptosis and induces G1/S cell-cycle progression; promotes EMT; supports tumor growth in vivo. |
| Molecular mechanism: |
Acts as a miR-7 sponge/antagonist, thereby de-repressing miR-7 target genes (EGFR, CCNE1, PIK3CD, RAF1) and modulating EMT markers (E-cadherin, N-cadherin). |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); cell cycle (promotes); migration (promotes); EMT (promotes); ceRNA regulation (promotes); EGFR (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; ROC Analysis; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; IF (Immunofluorescence); Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
Upregulated in OS tissues and shows diagnostic value (ROC AUC=0.857; cutoff 1.613); higher levels associated with high Enneking stage, tumor size, and pulmonary metastasis; predicted a poor clinical outcome. |
| Description: |
CDR1as is up-regulated in osteosarcoma and shows diagnostic potential. Functionally, it promotes OS cell proliferation/viability and migration and suppresses apoptosis and G1/S arrest by antagonizing miR-7 and de-repressing miR-7 targets (EGFR, CCNE1, PIK3CD, RAF1), also enhancing EMT marker changes. |
| Confidence score: |
0.7221 |