| Expression pattern: |
UP |
| Associated gene: |
WNT7B |
| Associated microRNA: |
miR-1182 |
| Biological function: |
Promotes OS progression (proliferation, cell cycle progression, migration, invasion, glycolysis) and inhibits apoptosis; knockdown represses tumor growth in vivo. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-1182 to upregulate WNT7B (ceRNA mechanism). |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); glycolysis (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; MTT; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_0105346 is associated with larger tumor size, higher Enneking stage, distant metastasis, and lower overall survival in OS patients. |
| Description: |
circ_0105346 is upregulated in osteosarcoma and functions as an oncogenic circRNA. It sponges miR-1182 to increase WNT7B expression, thereby promoting OS malignant behaviors (proliferation, migration/invasion, EMT-like changes, and glycolysis) and inhibiting apoptosis; its knockdown suppresses tumor growth in xenograft models and high expression correlates with worse overall survival. |
| Confidence score: |
0.7524 |