| Expression pattern: |
UP |
| Associated gene: |
AKT3, PI3K/AKT signaling pathway |
| Associated microRNA: |
miR-532-3p |
| Biological function: |
promotes proliferation, migration, invasion and tumor growth |
| Molecular mechanism: |
EV-delivered circNRIP1 acts as a miRNA sponge for miR-532-3p, relieving miR-532-3p-mediated repression of AKT3 and activating PI3K/AKT signaling |
| Biological pathway or process: |
PI3K/AKT (promotes); proliferation (promotes); migration (promotes); invasion (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Bioinformatics Analysis; FISH / smFISH; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Western Blot; In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
potential prognostic biomarker and therapeutic target in osteosarcoma |
| Description: |
In osteosarcoma, circNRIP1 is up-regulated and promotes malignant phenotypes (proliferation, migration, invasion) and tumor growth. It is delivered by BMSC-derived extracellular vesicles and functions as a sponge for miR-532-3p, thereby de-repressing AKT3 and activating the PI3K/AKT pathway. |
| Confidence score: |
0.7283 |