| Expression pattern: |
DN |
| Associated gene: |
PPP6C |
| Associated microRNA: |
miR-208a-3p |
| Biological function: |
inhibits proliferation, induces apoptosis, promotes autophagy, suppresses tumor growth/progression |
| Molecular mechanism: |
ceRNA mechanism: circCUL2 sponges miR-208a-3p to regulate PPP6C, modulating apoptosis and autophagy |
| Biological pathway or process: |
apoptosis (promotes); autophagy (promotes); proliferation (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Northern Blot; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; Cell Cycle Assay; IF (Immunofluorescence); Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
CircCUL2 would be an underlying target for the diagnosis and therapy of colorectal cancer. |
| Description: |
circCUL2 is down-regulated in colorectal cancer tissues and cell lines. Its overexpression suppresses CRC cell proliferation and tumor growth while inducing apoptosis and autophagy, acting through a ceRNA axis in which circCUL2 sponges miR-208a-3p to upregulate PPP6C. |
| Confidence score: |
0.7612 |