| Expression pattern: |
UP |
| Associated gene: |
c-Myc |
| Associated microRNA: |
miR-34c-5p |
| Biological function: |
Promotes NPC cell proliferation, migration, invasion, angiogenesis, and malignant phenotypes; acts as an oncogene in NPC cells. |
| Molecular mechanism: |
c-Myc activates circ-NOTCH1 by binding to the NOTCH1 promoter; circ-NOTCH1 functions as a ceRNA that sponges miR-34c-5p to upregulate c-Myc expression, forming a circ-NOTCH1/miR-34c-5p/c-Myc regulatory axis. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
RT-qPCR; FISH / smFISH; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; ChIP / ChIP-seq; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Tube Formation Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circ-NOTCH1 was overexpressed in NPC tissues and cells and may provide novel insight into the treatment of NPC. |
| Description: |
circ-NOTCH1 is up-regulated in nasopharyngeal carcinoma tissues and cell lines and functions as an oncogenic circRNA. c-Myc induces circ-NOTCH1 by binding the NOTCH1 promoter, while circ-NOTCH1 sponges miR-34c-5p to increase c-Myc expression, thereby promoting NPC cell proliferation, migration, invasion, and angiogenesis. |
| Confidence score: |
0.8344 |