| Expression pattern: |
DN |
| Associated gene: |
RUNX1 |
| Associated microRNA: |
miR-548g |
| Biological function: |
tumor suppressor; inhibits migration and invasion |
| Molecular mechanism: |
circFAT1 may sponge miR-548g, leading to increased miR-548g upon circFAT1 knockdown; downstream RUNX1 axis involvement in ESCC is unclear |
| Biological pathway or process: |
migration (inhibits); invasion (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; RT-qPCR; Transfection; Transwell Assay; Clinical Sample Validation; Survival Analysis |
| Clinical significance: |
High circFAT1 expression in tumor tissue is associated with better recurrence-free survival and cancer-specific survival and is an independent prognostic factor. |
| Description: |
circFAT1 is down-regulated in ESCC tissues and cell lines and acts as a tumor suppressor by inhibiting migration and invasion. Mechanistically, circFAT1 may sponge miR-548g (knockdown increases miR-548g), and higher tumor circFAT1 expression predicts better RFS/CSS in ESCC patients. |
| Confidence score: |
0.728 |