circRNA basic information
circBase ID: hsa_circ_0106800
Name: hsa_circ_PGAP3
Synonym: circ-PGAP3
Host Gene: PGAP3
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0002974
MONDO name: cervical cancer
Disease details: cervical cancer
Disease DO ID:
4362
Disease MeSH ID:
-
Disease NCIt ID:
C9311
Disease ICD11 ID:
1256072522
Disease OMIM ID:
603956
Species: Human
Species details: Homo sapiens
Tissue specimen:

CC tissues; normal control tissues; tumor tissues

Cell lines:

Hela; CaSKi; SiHa

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
DN
Associated gene: p53, p21, CDK4, Cyclin D1, Bcl2
Associated microRNA: miR-769-5p
Biological function: circ-PGAP3 acts as a tumor-inhibiting circRNA that reduces cervical cancer cell proliferation in vitro and retards tumor growth in vivo.
Molecular mechanism: circ-PGAP3 is transcriptionally elevated by p53 and functions as a cytoplasmic ceRNA sponge for miR-769-5p, relieving miR-769-5p-mediated repression of p53 and activating p53 signaling.
Biological pathway or process:

proliferation (inhibits); ceRNA regulation (promotes); p53 signaling (promotes); cell cycle (inhibits)

Detected method:
Q
S
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; ChIP / ChIP-seq; Transfection; CCK8; EdU Staining; Colony Formation Assay; Western Blot; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis

Clinical significance:

Low circ-PGAP3 expression was linked to poor prognosis, larger tumor size, advanced FIGO stage and lymph node metastasis; circ-PGAP3 may be a potential prognostic indicator and therapeutic target for cervical cancer.

Description:

circ-PGAP3 is downregulated in cervical cancer and low expression is associated with poor prognosis and aggressive clinicopathological features. Mechanistically, p53 transcriptionally induces circ-PGAP3, while cytoplasmic circ-PGAP3 sponges miR-769-5p to increase p53 signaling, thereby suppressing cervical cancer cell proliferation and tumor growth.

Confidence score:

0.8632

Other information
Title:

The novel circular RNA circ-PGAP3 retards cervical cancer growth by regulating the miR-769-5p/p53 axis.

Journal: Human cell
Published: 2021
PubMed ID: 33591461
Study type:

combined biological and clinical study

Data availability: three GEO databases on circRNA sequencing
Code availability: -