| Expression pattern: |
UP |
| Associated gene: |
SLC10A7, AGO2 |
| Associated microRNA: |
miR-296-3p |
| Biological function: |
Promotes glioma cell proliferation, invasion, tumor growth, tumorigenesis and invasiveness; circHECTD1 knockdown inhibits proliferation and invasion in vitro, decreases tumor growth and prolongs survival in vivo. |
| Molecular mechanism: |
circHECTD1 acts as a ceRNA/miRNA sponge that binds and suppresses miR-296-3p, resulting in upregulation of SLC10A7 and promotion of glioma progression. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNase R Treatment; RT-qPCR; RNA-seq; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Nuclear-Cytoplasmic Fractionation; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circHECTD1 expression predicts worse prognosis and is negatively correlated with overall survival in GBM patients; it may represent a novel therapeutic target for glioma progression. |
| Description: |
circHECTD1 is up-regulated in human glioma/GBM tissues and glioma cell lines, and high expression is associated with worse overall survival. Functionally, circHECTD1 promotes glioma cell proliferation, invasion and intracranial xenograft growth. Mechanistically, cytoplasmic circHECTD1 sponges miR-296-3p to relieve repression of SLC10A7, forming a circHECTD1/miR-296-3p/SLC10A7 regulatory axis. |
| Confidence score: |
0.8244 |