| Expression pattern: |
UP |
| Associated gene: |
TAB2 |
| Associated microRNA: |
miR-375 |
| Biological function: |
circ_AFF2 promotes FLS-RA cell cycle progression, proliferation and inflammatory response, and inhibits apoptosis in rheumatoid arthritis. |
| Molecular mechanism: |
circ_AFF2 acts as a sponge of miR-375 and upregulates TAB2 expression through the miR-375/TAB2 axis. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); inflammation (promotes); apoptosis (inhibits); cell cycle (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; Transfection; Cell Cycle Assay; MTT; Western Blot; Annexin V/PI Flow Cytometry; ELISA; RNA Pull-Down; Luciferase Reporter Assay; Bioinformatics Analysis |
| Clinical significance: |
Circ_AFF2 could be used as a biomarker in the diagnosis and treatment of RA and was considered as a potential target for RA diagnosis and treatment in the clinic. |
| Description: |
circ_AFF2 is up-regulated in RA blood samples and FLS-RA cells. It promotes cell cycle progression, proliferation and inflammatory cytokine production while suppressing apoptosis by sponging miR-375 and increasing TAB2 expression. The study proposes circ_AFF2 as a potential diagnostic and therapeutic target for rheumatoid arthritis. |
| Confidence score: |
0.7945 |