| Expression pattern: |
UP |
| Associated gene: |
SYF2 pre-mRNA splicing factor 2 |
| Associated microRNA: |
miR-621 |
| Biological function: |
promotes proliferation, migration, and invasion of gastric cancer cells |
| Molecular mechanism: |
acts as a miRNA sponge (ceRNA) for miR-621 to upregulate SYF2 |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; BrdU; Transwell Assay; Luciferase Reporter Assay; RNA Pull-Down; Western Blot; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
high circ_000064 expression was associated with a worse overall survival time of patients with GC; high level of circ_000064 was positively correlated with lymph node metastasis and advanced TNM stage |
| Description: |
circ_0000064 is up-regulated in gastric cancer and promotes GC cell proliferation, migration, and invasion. Mechanistically, it sponges miR-621 to relieve repression of SYF2, forming a circ_0000064/miR-621/SYF2 ceRNA axis, and high expression is associated with worse overall survival. |
| Confidence score: |
0.7779 |