circRNA basic information
circBase ID: -
Name: hsa_circ_GFRA1
Synonym: circGFRA1
Host Gene: GFRA1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005494
MONDO name: triple-negative breast carcinoma
Disease details: triple-negative breast cancer
Disease DO ID:
 0060081
Disease MeSH ID:
 D064726
Disease NCIt ID:
 C71732
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumour tissues
Cell lines:

MDA-MB-231; MDA-MB-231.PR; 293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: TLR4
Associated microRNA: miR-361-5p
Biological function: Promotes paclitaxel resistance in TNBC cells; circGFRA1 knockdown increases PTX sensitivity, inhibits cell viability and promotes apoptosis under PTX treatment.
Molecular mechanism: circGFRA1 functions as a miR-361-5p sponge and regulates TLR4 expression, thereby affecting TNBC cell resistance to paclitaxel.
Biological pathway or process:

chemoresistance (promotes); drug resistance (promotes); apoptosis (inhibits); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; Bioinformatics Analysis
Clinical significance:

-
Description:

circGFRA1 is up-regulated in paclitaxel-resistant TNBC MDA-MB-231.PR cells and promotes resistance to paclitaxel. Mechanistically, circGFRA1 acts as a sponge for miR-361-5p, leading to increased TLR4 expression; circGFRA1 knockdown restores miR-361-5p, reduces TLR4, inhibits cell viability, promotes apoptosis, and enhances PTX sensitivity in vitro and in xenograft tumors.
Confidence score:

0.657
Other information
Title:

circGFRA1 affects the sensitivity of triple-negative breast cancer cells to paclitaxel via the miR-361-5p/TLR4 pathway.

Journal: Journal of biochemistry
Published: 2021
PubMed ID: 33481008
Study type:

biological research
Data availability: Supplementary Data are available at JB Online
Code availability: -