circRNA basic information
circBase ID: hsa_circ_0000231
Name: hsa_circ_ARHGAP12
Synonym: circular RNA_0000231
Host Gene: ARHGAP12
Genomic location(hg19): chr10:32197099-32199491:-
Genomic location(hg38): chr10:31908171-31910563:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005311
MONDO name: atherosclerosis
Disease details: atherosclerosis
Disease DO ID:
 1936
Disease MeSH ID:
 D050197
Disease NCIt ID:
 C35768
Disease ICD11 ID:
 109367356
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

HUVECs
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: CLIC4, ARHGAP12
Associated microRNA: miR-135a-5p
Biological function: Circ_0000231 contributed to ox-LDL-induced injury of HUVECs, antagonized the protective role of ASV, reduced cell viability and migration, and promoted apoptosis, inflammatory response, oxidative stress, MCP1 and ICAM1 expression.
Molecular mechanism: Circ_0000231 acts as a miRNA sponge for miR-135a-5p and regulates CLIC4 expression through the circ_0000231/miR-135a-5p/CLIC4 axis in ox-LDL-induced HUVECs.
Biological pathway or process:

ceRNA regulation (other); apoptosis (promotes); migration (inhibits); inflammation (promotes); other pathway/process (promotes)
Detected method:
Q
Validation methods:

Actinomycin D / DRB Stability Assay; RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Transfection; MTT; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; ELISA; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; Bioinformatics Analysis
Clinical significance:

-
Description:

In an ox-LDL-induced HUVEC atherosclerosis cell model, circ_0000231 was up-regulated and promoted endothelial cell injury, including apoptosis, inflammation, oxidative stress and impaired migration/viability. Mechanistically, circ_0000231 sponged miR-135a-5p to regulate CLIC4, and ASV alleviated cell injury by targeting the circ_0000231/miR-135a-5p/CLIC4 axis.
Confidence score:

0.763
Other information
Title:

Astragaloside IV alleviates atherosclerosis through targeting circ_0000231/miR-135a-5p/CLIC4 axis in AS cell model in vitro.

Journal: Molecular and cellular biochemistry
Published: 2021
PubMed ID: 33439448
Study type:

biological research
Data availability: -
Code availability: -