| Expression pattern: |
UP |
| Associated gene: |
KPNA4 |
| Associated microRNA: |
miR-195-5p |
| Biological function: |
promotes paclitaxel resistance, proliferation, migration and invasion; inhibits apoptosis; promotes cell cycle progression in PTX-resistant NSCLC cells |
| Molecular mechanism: |
circ_ZFR acts as a miRNA sponge for miR-195-5p to upregulate KPNA4, thereby enhancing PTX resistance and malignant phenotypes |
| Biological pathway or process: |
chemoresistance (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; MTT; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
circ_ZFR is highly expressed in PTX-resistant NSCLC tissues compared with PTX-sensitive and normal tissues |
| Description: |
circ_ZFR is upregulated in paclitaxel-resistant NSCLC tissues and PTX-resistant cell models. It promotes chemoresistance and malignant behaviors by sponging miR-195-5p and thereby increasing KPNA4 expression; circ_ZFR knockdown restores PTX sensitivity in vitro and in a xenograft model. |
| Confidence score: |
0.7228 |