| Expression pattern: |
UP |
| Associated gene: |
ATG5 |
| Associated microRNA: |
miR-149-5p |
| Biological function: |
Circ-FOXM1 promotes NSCLC cell viability, migration, autophagy and tumor growth, and suppresses apoptosis; circ-FOXM1 knockdown represses NSCLC development. |
| Molecular mechanism: |
Circ-FOXM1 acts as a competing endogenous RNA sponge for miR-149-5p, thereby upregulating ATG5 expression and promoting NSCLC progression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); autophagy (promotes); apoptosis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; Luciferase Reporter Assay; RNA Pull-Down; Bioinformatics Analysis |
| Clinical significance: |
Circ-FOXM1 expression was higher in advanced TNM stage NSCLC patients, suggesting a potential therapeutic target for NSCLC. |
| Description: |
Circ-FOXM1 is upregulated in NSCLC tissues and cells and is associated with advanced TNM stage. Functionally, circ-FOXM1 promotes NSCLC cell viability, migration, autophagy and tumor growth while suppressing apoptosis. Mechanistically, circ-FOXM1 sponges miR-149-5p to increase ATG5 expression, forming a circ-FOXM1/miR-149-5p/ATG5 regulatory axis. |
| Confidence score: |
0.7591 |