| Expression pattern: |
UP |
| Associated gene: |
SMAD4 |
| Associated microRNA: |
miR-574-5p |
| Biological function: |
hsa_circ_0001320 was upregulated in keloid tissues and may be related to keloid formation and development; it may regulate SMAD4 through miR-574-5p sponge adsorption and affect keloid occurrence and development through the TGF-beta pathway. |
| Molecular mechanism: |
ceRNA mechanism involving hsa_circ_0001320 binding miR-574-5p and possibly regulating SMAD4 through the TGF-beta pathway |
| Biological pathway or process: |
ceRNA regulation (other); Wnt/beta-catenin (other); TGF-beta/SMAD (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNase R Treatment; RT-qPCR; circRNA-seq; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; Bioinformatics Analysis |
| Clinical significance: |
Potential novel therapeutic target or diagnostic biomarker for keloid patients |
| Description: |
hsa_circ_0001320 is significantly upregulated in keloid tissue and its expression was validated by QPCR in an expanded clinical cohort. The study confirmed direct interaction between hsa_circ_0001320 and miR-574-5p by dual-luciferase reporter assay, and proposed that it may influence keloid development through a miR-574-5p/SMAD4/TGF-beta-related mechanism. |
| Confidence score: |
0.6902 |