| Expression pattern: |
UP |
| Associated gene: |
TRAF2 |
| Associated microRNA: |
miR-671-5p (miR-671), miR-671 |
| Biological function: |
Promotes IL-1beta-induced chondrocyte injury; knockdown enhances cell viability, suppresses inflammation, and inhibits apoptosis in IL-1beta-induced CHON-001 cells. |
| Molecular mechanism: |
circ_0114876 acts as a ceRNA by sponging miR-671 to regulate TRAF2 expression, thereby modulating cell progression and inflammation in IL-1beta-induced CHON-001 cells. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (inhibits); apoptosis (promotes); inflammation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; ELISA; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Potential diagnostics and therapeutics in OA; circ_0114876 is described as an important biomarker related to treatment of OA. |
| Description: |
circ_0114876 is up-regulated in OA tissues and IL-1beta-induced CHON-001 chondrocytes. It promotes IL-1beta-induced chondrocyte injury by sponging miR-671 and increasing TRAF2 expression, thereby reducing cell viability and promoting apoptosis and inflammatory responses. The study suggests circ_0114876 may be a potential biomarker and therapeutic target in OA. |
| Confidence score: |
0.8111 |