| Expression pattern: |
UP |
| Associated gene: |
PUM2 |
| Associated microRNA: |
miR-758-3p |
| Biological function: |
Promotes cervical cancer cell proliferation, migration, invasion, glycolysis and tumor growth, and inhibits apoptosis. |
| Molecular mechanism: |
circRBM33 functions as a ceRNA/miRNA sponge for miR-758-3p to upregulate PUM2 expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); EMT (promotes); glycolysis (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Microarray; Clinical Sample Validation; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Transfection; MTT; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circRBM33 expression was associated with tumor size, FIGO stage, lymph node metastases and shorter overall survival, suggesting prognostic biomarker potential. |
| Description: |
circRBM33 is upregulated in cervical cancer tissues and cells, and high expression is associated with larger tumor size, advanced FIGO stage, lymph node metastasis and shorter overall survival. Functionally, circRBM33 promotes cervical cancer proliferation, migration, invasion, glycolysis and xenograft tumor growth while suppressing apoptosis. Mechanistically, it acts as a ceRNA by sponging miR-758-3p to increase PUM2 expression. |
| Confidence score: |
0.8508 |