| Expression pattern: |
UP |
| Associated gene: |
E2F3, PGPEP1, Bcl-2, Bax, cleaved PARP |
| Associated microRNA: |
miR-1297 |
| Biological function: |
circ_PGPEP1 promotes GC cell cycle progression, proliferation, migration, invasion, metastasis, and tumor growth, and inhibits apoptosis. |
| Molecular mechanism: |
circ_PGPEP1 functions as a ceRNA by sponging miR-1297 to positively regulate E2F3 expression, thereby promoting GC progression. |
| Biological pathway or process: |
ceRNA regulation (promotes); cell cycle (promotes); proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Colony Formation Assay; MTT; Transwell Assay; Western Blot; In Vivo Animal Model |
| Clinical significance: |
High circ_PGPEP1 expression was related to TNM stage and metastasis of GC patients; circ_PGPEP1 might serve as a potential biomarker for GC. |
| Description: |
circ_PGPEP1 is up-regulated in gastric cancer tissues and cell lines and is associated with TNM stage and metastasis. Functionally, it promotes GC cell proliferation, cell cycle progression, migration, invasion, and xenograft tumor growth while suppressing apoptosis. Mechanistically, circ_PGPEP1 acts as a cytoplasmic ceRNA that sponges miR-1297 to increase E2F3 expression. |
| Confidence score: |
0.78 |