circRNA basic information
circBase ID: hsa_circ_0001721
Name: hsa_circ_CDK14
Synonym: circCDK14
Host Gene: CDK14
Genomic location(hg19): chr7:90355880-90356126:+
Genomic location(hg38): chr7:90726566-90726812:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0021042
MONDO name: glioma
Disease details: glioma
Disease DO ID:
 -
Disease MeSH ID:
 D005910
Disease NCIt ID:
 C3059
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

glioma tissue; non-tumor brain tissue
Cell lines:

HEB; U251; U87; SF126
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PDGFRA
Associated microRNA: miR-3938
Biological function: Promotes proliferation, migration, invasion and tumorigenesis in glioma; reduces glioma cells' sensitivity to erastin-induced ferroptosis.
Molecular mechanism: Acts as a ceRNA by sponging miR-3938 to upregulate PDGFRA; modulates PDGFRalpha signaling affecting GPX4 and SLC7A11 and thereby ferroptosis sensitivity; promotes EMT markers via PDGFRA.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ferroptosis (inhibits); ceRNA regulation (promotes)
Detected method:
Q
S
Validation methods:

RNA-seq; Bioinformatics Analysis; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Wound Healing Assay; Transwell Assay; Western Blot; In Vivo Animal Model; Survival Analysis; Clinical Sample Validation
Clinical significance:

Elevated circCDK14 levels are associated with poor overall survival (OS) of glioma patients.
Description:

circCDK14 (hsa_circ_0001721), derived from CDK14, is up-regulated in glioma tissues/cell lines and its high expression predicts worse OS. Functionally, it promotes glioma proliferation, migration, invasion and xenograft growth. Mechanistically, circCDK14 mainly localizes in the cytoplasm and sponges miR-3938 to increase PDGFRA, thereby enhancing malignant behavior and decreasing sensitivity to erastin-induced ferroptosis, partly via PDGFRalpha signaling affecting GPX4/SLC7A11.
Confidence score:

0.8658
Other information
Title:

CircCDK14 Promotes Tumor Progression and Resists Ferroptosis in Glioma by Regulating PDGFRA.

Journal: International journal of biological sciences
Published: 2022
PubMed ID: 35002529
Study type:

combined biological and clinical study
Data availability: -
Code availability: -