circRNA basic information
circBase ID: hsa_circ_0005660
Name: hsa_circ_NFIX
Synonym: circNFIX
Host Gene: NFIX
Genomic location(hg19): chr19:13135834-13136366:+
Genomic location(hg38): chr19:13025020-13025552:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0006373
MONDO name: pituitary gland adenoma
Disease details: pituitary adenoma
Disease DO ID:
3829
Disease MeSH ID:
-
Disease NCIt ID:
C3329
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

pituitary adenoma tissues

Cell lines:

GT1-1; GH3; HEK-293T

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: CCNB1
Associated microRNA: miR-34a-5p
Biological function: circNFIX promotes pituitary adenoma cell invasion, migration, proliferation and tumor formation; circNFIX silencing inhibits these phenotypes.
Molecular mechanism: circNFIX acts as a ceRNA/miRNA sponge for miR-34a-5p, relieving suppression of CCNB1 and thereby promoting pituitary adenoma progression.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; Transwell Assay; CCK8; Colony Formation Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model

Clinical significance:

High circNFIX expression is related to high Knosp grade and poor prognosis, suggesting circNFIX is a potential biomarker and treatment target for pituitary adenomas.

Description:

circNFIX (hsa_circ_0005660) is up-regulated in invasive pituitary adenomas and promotes invasion, migration, proliferation, and tumor formation. Mechanistically, it sponges miR-34a-5p to up-regulate CCNB1, forming a circNFIX/miR-34a-5p/CCNB1 axis. High circNFIX expression is associated with high Knosp grade and poor prognosis, suggesting potential biomarker and therapeutic relevance.

Confidence score:

0.7126

Other information
Title:

CircNFIX promotes progression of pituitary adenoma via CCNB1 by sponging miR-34a -5p.

Journal: Molecular and cellular endocrinology
Published: 2021
PubMed ID: 33359304
Study type:

combined biological and clinical study

Data availability: -
Code availability: -