| Expression pattern: |
UP |
| Associated gene: |
AKT3 |
| Associated microRNA: |
miR-1258 |
| Biological function: |
promotes NSCLC cell growth, migration, invasion, and tumor growth |
| Molecular mechanism: |
circ_0000520 acts as a miRNA sponge for miR-1258 to upregulate AKT3 |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); PI3K/AKT (other); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Western Blot; In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Highly expressed circ_0000520 is linked to the NSCLC patient's advanced TNM stage and lymph node metastasis; high circ_0000520 expression was positively correlated with low overall survival rate |
| Description: |
circ_0000520 is upregulated in NSCLC and promotes malignant phenotypes (proliferation, migration, invasion) and tumor growth. Mechanistically, it functions as a ceRNA by sponging miR-1258 to increase AKT3 expression, and higher circ_0000520 is associated with advanced TNM stage, lymph node metastasis, and poorer overall survival. |
| Confidence score: |
0.7486 |